Hi there,
The answer to your first question is yes, grain size is a factor affecting the flexibility of copper wire. Unfortunately it is only one of many variables and I have never personally come across a technical paper relating grain size to the mechanical properties of a particular diameter of copper wire. Perhaps others will give us more information.
There was a paper in the October 1991 (Page 91) edition of The Wire Journal International titled "The Effects of Deformation and Annealing Conditions on the Flexibility of Copper Wire" by Y.T. Kim, J.Y. Jang, C.H. Kang, and K.H. Hahn, all of GoldStar Cable in Korea. It focused on bare copper wire destined to become magnet wire.
The header for the paper states "The amount of deformation before annealing should not exceed a critical value, because severely deformed copper wire is hard to recrystalize."
I recommend you purchase a reprint of this paper and carefully read it. You can order a copy of it by sending an E-mail with the full title of the paper including year, month and page number to
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Kindest regards,
Peter J. Stewart-Hay
Principal
Stewart-Hay Associates
www.Stewart-Hay.com